Rapid identification of Phytophthora ramorum using PCR-SSCP analysis of ribosomal DNA ITS-1

Rapid identification of Phytophthora ramorum using PCR-SSCP analysis of ribosomal DNA ITS-1.

AIMS The primary objectives of this study were to determine if a single-strand conformation polymorphism (SSCP) analysis can be used for rapid identification of Phytophthora ramorum, an important quarantine plant pathogen worldwide, and to further assess the potential of the SSCP technique as a taxonomic tool for the genus Phytophthora. METHODS AND RESULTS SSCP of ribosomal DNA internal trans...

Species identification of five penaeid shrimps using PCR-RFLP and SSCP analyses of 16S ribosomal DNA.

DNA-based molecular markers for differentiation of five penaeid shrimps (Penaeus monodon, P. semisulcatus, Feneropenaeus merguiensis, Litopenaeus vannamei and Marsupenaeus japonicus) were developed based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single-stranded conformation polymorphism (SSCP) of 16S ribosomal (r) DNA. Differentiation of P. monodon, P....

Molecular Identification and Detection of Phytophthora ramorum

The genus Phytophthora comprises over 70 described species, however many new species have been reported recently as a result of the discovery of previously undetected species or by the hybridization of known species. Phytophthora ramorum, one of the new Phytophthora species, is considered a high phytosanitary risk because it is currently responsible for largescale oak mortality in coastal Calif...

Phytophthora ramorum

comparative dna interaction studies of antiviral drug, zidovudine and its complex using different instrumental methods

هدف از این مطالعه بررسی امکان استفاده از داروهای شناخته شده در درمان سایر بیماریها به عنوان داروهای ضد سرطان است. همچنین با استفاده از این داروها در ساختمان کمپلکس فلز می توان شاخص های دارویی بدست آمده را بررسی نمود. داروی ضد ویروس ایدز(hiv)به نام زیدوودین(azt)انتخاب و.کمپلکس.محلول.در.آب[pt(azt)2]cl2سنتزو به روشهای مختلف فیزیکی و شیمیایی شناسایی گردید. بر هم کنش مقایسه ای این دارو و کمپلکس پلا...